What absorbs at 3250 cm⁻¹ in an FTIR spectrum?
A band near 3250 cm⁻¹ can point to several functional groups. Below are the most likely assignments, ranked by how much published evidence supports each — every one traceable to literature (DOI) and cross-validated against our 130,000+ reference spectra and knowledge graph.
Backed by 8 cited sources
Possible functional-group assignments
| Functional group | Supporting facts | Cited sources | Top confidence |
|---|---|---|---|
| Hydroxyl (O-H) | 14 | 13 | 1.0 |
| N h | 5 | 4 | 1.0 |
| Water (H2O) | 1 | 1 | 0.9 |
| Hydrogen bond | 1 | 1 | 0.8 |
Ranking reflects accumulated literature evidence, not a single authoritative rule. Always confirm against your sample context.
Literature behind these assignments
-
N h confidence 1.0
“Characteristic peak of valdecoxib”
Thakral 等 - 2010 - Eudragit S-100 entrapped chitosan microspheres of DOI: 10.1007/s10856-010-4109-2 -
Hydroxyl (O-H) confidence 1.0
“Explicit assignment in text: 'bands located at 3250 cm-1 ... due to O-H stretching'”
Ducrozet 等 - 2021 - New Insights into the Reactivity of Detonation Nan DOI: 10.3390/nano11102671 -
confidence 1.0
“Assigned to asymmetric stretching vibrations of NH bonds from DEA.”
Vasconcelos 等 - 2012 - Optical characterization of 316L stainless steel c DOI: 10.1016/j.jnoncrysol.2012.07.035 -
Water (H2O) confidence 0.9
“Broad band centred around 3250 cm-1 attributed to water.”
Asghar 等 - 2020 - Ethylenediamine loading into a manganese-based met DOI: 10.1098/rsos.191934 -
Hydroxyl (O-H) confidence 0.9
“Explicit assignment in text.”
Dennis 等 - 2022 - Substantial Proton Ion Conduction in Methylcellulo DOI: 10.3390/membranes12070706 -
confidence 0.9
“Explicit assignment in text.”
Eylul Kiymaci 等 - 2022 - Antioxidative, cytotoxic, and antibacterial proper DOI: 10.2478/aiht-2022-73-3658 -
N h confidence 0.9
“Explicit assignment in text”
Kamboj 和 Verma - 2019 - Palmitic Acid-Pluronic F127-Palmitic Acid Pentablo DOI: 10.4274/tjps.galenos.2018.20082 -
Hydroxyl (O-H) confidence 0.9
“OH-stretching vibrations”
Li 等 - 2021 - Long-Range Structures of Amorphous Solid Water DOI: 10.1021/acs.jpcb.1c06899
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