What absorbs at 3297 cm⁻¹ in an FTIR spectrum?
A band near 3297 cm⁻¹ can point to several functional groups. Below are the most likely assignments, ranked by how much published evidence supports each — every one traceable to literature (DOI) and cross-validated against our 130,000+ reference spectra and knowledge graph.
Backed by 8 cited sources
Possible functional-group assignments
| Functional group | Supporting facts | Cited sources | Top confidence |
|---|---|---|---|
| N h | 5 | 4 | 1.0 |
| Hydroxyl (O-H) | 3 | 3 | 1.0 |
| Amide | 3 | 3 | 1.0 |
| Secondary amine | 2 | 1 | 1.0 |
| Protein | 1 | 1 | 1.0 |
Ranking reflects accumulated literature evidence, not a single authoritative rule. Always confirm against your sample context.
Literature behind these assignments
-
Amide confidence 1.0
“The band at 3297 is assigned to amide”
Gupta 等 - 2022 - In vitro cell composition identification of wood d DOI: 10.1098/rsos.201935 -
Amide confidence 1.0
“The amide A band was located at 3297 associated with cm-1 the stretch vibration of the N-H group.”
Green Synthesis of Silver Nanoparticles for Preparation of Gelatin Films with Antimicrobial Activity DOI: 10.3390/polym14173453 -
confidence 1.0
“sin4 1.81 N-H bend 1179 137 N-H(cid:5)(cid:5)(cid:5) stretching 3297 263 N-H stretching 3343”
Ay 和 Aydinli - 2004 - Comparative investigation of hydrogen bonding in s DOI: 10.1016/j.optmat.2003.12.004 -
Secondary amine confidence 1.0
“and N-H stretching of primary and secondary amines at 3297 Amide bands are: C=O”
Haramina 等 - 2019 - EFFECT OF WEATHERING ON DYNAMIC MECHANICAL PROPERT DOI: 10.21278/TOF.43401 -
Hydroxyl (O-H) confidence 1.0
“cm-1, Analysing Dragon's Blood, Figure 4, there were bands at 3297 possibly due to OH groups vibracm-1 cm-1, tions [20];”
Oliveira 等 - 2016 - FTIR analysis and quantification of phenols and fl DOI: 10.1590/S1517-707620160003.0072 -
Hydroxyl (O-H) confidence 1.0
“The N-H deformation and C-N stretchin PVA, with the main peako bserved in FTIR spectra at ing vibrations (amide II) of peptides, enzymes, and other cm-1 cm-1 wavenumbers 3297 (O-H stretching) and 2922 hemolymph protein-based constituents oc”
Nigella/honey/garlic/olive oil co-loaded PVA electrospun nanofibers for potential biomedical applications DOI: 10.1007/s40204-022-00207-5 -
N h confidence 0.9
“N-H vibration”
Comparison of Different Methods for Spongin-like Collagen Extraction from Marine Sponges (Chondrilla caribensis and Aplysina fulva): Physicochemical Properties and In Vitro Biological Analysis DOI: 10.3390/membranes11070522 -
Hydroxyl (O-H) confidence 0.8
“Peak at 3297 attributed to O-H stretch”
El Mohtadi 等 - 2020 - DIFFERENTIAL ENGULFMENT OF STAPHYLOCOCCUS AUREUS A DOI: 10.17179/excli2020-2766
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